A CONGO RED STAINING METHOD FOR EPOXY-EMBEDDED AMYLOID

Detecting microcalcifications in atherosclerotic plaques by a simple trichromic staining method for epoxy embedded carotid endarterectomies

Atherosclerotic plaques have a high probability of undergoing rapid progression to stenosis, becoming responsible of acute coronary syndrome or stroke. Microcalcifications may act as enhancers of atherosclerotic plaque vulnerability. Considering that calcifications with a diameter smaller than 10 mm in paraffin embedded tissue are rather difficult to detect, our aim was to analyze microcalcific...

Is Congo red an amyloid-specific dye?

Congo red (CR) binding, monitored by characteristic yellow-green birefringence under crossed polarization has been used as a diagnostic test for the presence of amyloid in tissue sections for several decades. This assay is also widely used for the characterization of in vitro amyloid fibrils. In order to probe the structural specificity of Congo red binding to amyloid fibrils we have used an in...

Caught Red Handed- Congo Staining in the Oropharynx

Selective amyloid staining as a function of amyloid composition and structure. Histochemical analysis of the alkaline Congo red, standardized toluidine blue, and iodine methods.

Microcalcifications as seen in epoxy-embedded carotids using a trichromic staining

Atherosclerotic plaques that undergo rapid progression to stenosis are responsible of stroke or acute coronary syndrome. The vulnerability of the atherosclerotic plaque may be enhanced due to the presence of microcalcifications. Our aim was to analyze microcalcifications on epoxy resin-embedded semithin sections from bioptic samples of carotid endarterectomies. An original trichromic method con...